Journal: Cancer reports (Hoboken, N.J.)

Article Title: Forced Vasohibin-1 Expression Increases Paclitaxel Sensitivity of Ovarian Cancer by Inhibiting Microtubule Activity.

doi: 10.1002/cnr2.70100

Figure Lengend Snippet: FIGURE 2 | Western blotting of cyclin B1 in VASH1-overexpressing or control luciferase gene-transfected ovarian cancer cell lines. (A) Cyclin B1 expression was weakly detected in control cells (SKOV-3/LUC and SHIN-3/LUC), whereas SKOV-3/VASH1 and SHIN-3/VASH1 cells clearly ex- pressed cyclin B1 at the position corresponding to a molecular weight of 55 kDa. β-actin in the cell lysate was used as a loading control. Each number indicates the relative protein expression level compared with β-actin as 1.0. (B) Densitometric analysis is shown in the column graph. Data are shown as the mean and SD. *p < 0.05 (vs. LUC gene transfected cells). LUC: luciferase, VASH: vasohibin.

Article Snippet: Antibody Catalog no. Company Anti- detyrosinated alpha tubulin rabbit polyclonal antibody ab48389 Abcam, Cambridge, UK Anti- alpha tubulin mouse monoclonal antibody sc- 32 293 Santa Cruz Biotechnology Inc., Dallas, TX, USA Anti- cyclin B1 rabbit monoclonal antibody 12 231 Cell Signaling Technology Inc., Danvers, MA, USA Anti- β- actin rabbit polyclonal antibody A2066 Sigma- Aldrich; Merck KGaA nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cnr2.70100 by loki revolution - C A PE S , W iley O nline L ibrary on [25/12/2024].

Techniques: Western Blot, Control, Luciferase, Transfection, Expressing, Molecular Weight

Journal: eLife

Article Title: Systematic lncRNA mapping to genome-wide co-essential modules uncovers cancer dependency on uncharacterized lncRNAs

doi: 10.7554/eLife.77357

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-cyclin B1 (mouse monoclonal) , BD Pharmingen , #554177 , WB (1:1000).

Techniques: Transfection, Construct, Plasmid Preparation, Staining, Sequencing, SYBR Green Assay, Isolation, Proliferation Assay, Colony Assay, Cell Cycle Assay, Positive Control, Software

Journal: PLoS ONE

Article Title: Sterigmatocystin-Induced DNA Damage Triggers G 2 Arrest via an ATM/p53-Related Pathway in Human Gastric Epithelium GES-1 Cells In Vitro

doi: 10.1371/journal.pone.0065044

Figure Lengend Snippet: In response to ST-induced DNA damage, ATM serves as a signal transducer for the activation of its downstream signaling pathway. Activated ATM simultaneously phosphorylates the Thr-68 and Ser-15 residues of Chk2 and p53, respectively. These phosphorylations lead to the activation of their downstream pathway components, which results in the inhibition of the activation of Cdc25 and an increase in the expression of p21 waf1 . These steps finally result in the inactivation of the Cyclin B1/Cdc2 complex and the induction of G 2 arrest.

Article Snippet: The primary antibodies used for the Western blot analysis were mouse anti-human Cyclin B1 antibody (eBioscience, CA, USA), rabbit anti-human Cdc2, Cdc25C, ATM, phospho-ATM (Ser-1981), and phospho-Chk2 (Thr-68) monoclonal antibodies (Epitomics, CA, USA), rabbit anti-human Chk2 monoclonal antibodies (Millipore, MA, USA), rabbit anti-human phospho-Cdc2 (Tyr15) and phospho-Cdc25C (Ser216) monoclonal antibodies (Cell Signaling Technology, MA, USA), mouse anti-human phospho-p53 (Ser15) monoclonal antibody (Cell Signaling Technology, MA, USA), rabbit anti-human p53, Bax, and caspase-3 antibodies, and mouse anti-human p21 waf1 and Bcl-2 antibodies (Santa Cruz, CA, USA).

Techniques: Activation Assay, Inhibition, Expressing